Abstract
ABSTRACTBlue mold is the vernacular name of a common postharvest disease of stored apples, pears, and quince that is caused by several common species of Penicillium. This study reports the draft genome sequence of Penicillium expansum strain R21, which was isolated from a red delicious apple in 2011 in Pennsylvania.
Highlights
Blue mold is the vernacular name of a common postharvest disease of stored apples, pears, and quince that is caused by several common species of Penicillium
Spores of P. expansum R21 isolated in 2011 from a decomposing red delicious apple in Pennsylvania were inoculated in potato dextrose broth and incubated with shaking at 200 rpm at 25°C for 7 days
Annotation results indicate that the P. expansum R21 contains 12,707 predicted genes, with an average length of 1,478 bp
Summary
Blue mold is the vernacular name of a common postharvest disease of stored apples, pears, and quince that is caused by several common species of Penicillium. Spores of P. expansum R21 isolated in 2011 from a decomposing red delicious apple in Pennsylvania were inoculated in potato dextrose broth and incubated with shaking at 200 rpm at 25°C for 7 days. Total genomic DNA was extracted using an E.Z.N.A. Fungal DNA midi kit (Omega Bio-Tek) according to the manufacturer’s instructions. Three DNA libraries with different inserted size (paired-end 410-bp, mate pair 2kbpϩ8kbp) of P. expansum R21 were sequenced using an Illumina MiSeq benchtop sequencer and the sequence depths reached 105ϫ, 109ϫ, and 84ϫ, respectively.
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