Abstract
Plant growth-promoting bacteria are ecological alternatives for fertilization, mainly for gramineous. Since plant x bacteria interaction is genotype and strain dependent, searching for new strains may contribute to the development of new biofertilizers. We aim to characterize plant growth-promoting capacity of Leclercia adecarboxylata strain Palotina, formerly isolated by our group in corn. A single isolated colony was taken and its genome was sequenced using Illumina technology. The whole genome was compared to other Leclercia adecarboxylata strains, and their biological and growth-promoting traits, such as P solubilization and auxin production, were tested. Following that, a 4.8 Mb genome of L. adecarboxylata strain Palotina was assembled and the functional annotation was carried out. This paper is the first to report the genes associated with plant growth promotion demonstrating in vitro indole acid production by this strain. These results project the endophyte as a potential biofertilizer for further commercial exploitation.
Highlights
Leclercia adecarboxylata, a member of the Enterobacteriaceae family, is a motile, aerobic, omnipresent Gram-negative bacterium
The genome of Leclercia adecarboxylata strain Palotina was represented in 20 contigs, sized 4,801,735 bp, with GC content of 55.7%, 4.379 coding sequences and no plasmid were observed
Group genes related to bacterial systems, such as several hypothetical proteins, Type I restriction and cobalt/cadmium/zinc RND efflux, were absent in all strains used in the comparison (Figure 1, Figures S1 to S3)
Summary
A member of the Enterobacteriaceae family, is a motile, aerobic, omnipresent Gram-negative bacterium. Infections by L. adecarboxylata in humans are scarcely reported, being considered an opportunistic pathogen (Kashani et al, 2014; Hoyos-Mallecot et al, 2017; Choudhary et al, 2018) This strain was first described and named Escherichia adecarboxylata by Leclerc (1962), and, later received the generic name Leclercia from Tamura et al, (1986). It was phenotypically differentiated by biochemical and DNA hybridization assays from other Enterobacteriaceae species (Choudhary et al, 2018). These regulators mainly promote central and lateral root growth, increasing the absorption surface, which in turn increases the root’s nutrient and water uptake (Beneduzi et al, 2012; Jha and Saraf, 2015)
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