Abstract
Bacteria within the genus Alcaligenes, exhibit diverse properties but remain largely unexplored at genome scale. To shed light on the genome structure, heterogeneity and traits of Alcaligenes species, the genome of a tannery effluent isolated Alcaligenes faecalis subsp. phenolicus MB207 was sequenced and assembled. The genome was compared to the whole genome sequences of genus Alcaligenes present in the National Centre for Biotechnology Information database. Core, pan and species specific gene sequences i.e. singletons were identified. Members of this genus did not portray exceptional genetic heterogeneity or conservation and out of 5,166 protein coding genes from pooled genome dataset, 2429 (47.01%) contributed to the core, 1193 (23.09%) to singletons and 1544 (29.88%) to accessory genome. Secondary metabolite forming apparatus, antibiotic production and resistance was also profiled. Alcaligenes faecalis subsp. phenolicus MB207 genome consisted of a copious amount of bioremediation genes i.e. metal tolerance and xenobiotic degrading genes. This study marks this strain as a prospective eco-friendly bacterium with numerous benefits for the environment related research. Availability of the whole genome sequence heralds an opportunity for researchers to explore enzymes and apparatus for sustainable environmental clean-up as well as important compounds/substance production.
Highlights
Alcaligenes specie strains exist in soil, water, and environment, as well as in association with humans
Genome assembly through IDBA-UD approach resulted in 9 scaffolds
The sequence reads have been deposited in the NCBI SRA database and allocated the accession number: SRR5809679
Summary
Alcaligenes specie strains exist in soil, water, and environment, as well as in association with humans The bacteria of this genus are usually non-pathogenic but occasional opportunistic infections could occur in humans. Nanoparticle production[11], nematicidal[12] and biocontrol activity[13] has been reported in addition to production of chemicals[14], detergent[15], gum[16], and bioplastics[17]. Despite such high applicability of Alcaligenes species in major spheres of research and prospective benefits in industry, agricultural and environmental domain, it remains underrepresented and understudied at whole genome level. Our group is further working on the various molecular aspects of this bacterium both in vitro and in silico, to further shed light on the mechanisms beneath its bioremediation capability
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