Abstract
Here, we describe nearly complete genome sequences (7,361 nucleotides [nt] and 6,893 nt) of two echovirus 20 (E20) isolates from Nigeria that were simultaneously typed as CVB and E20 (dual serotype) by neutralization assay. Both include two overlapping open reading frames (ORFs) of 67 and 2,183 amino acids that encoded a recently described gut infection-facilitating protein and the classic enterovirus proteins, respectively.
Highlights
Enteroviruses (EVs) are members of the genus Enterovirus, family Picornaviridae, and order Picornavirales
All software was used with default settings
The missing region was recovered by reverse transcription-PCR (RT-PCR) with specific primers and Sanger sequencing
Summary
Enteroviruses (EVs) are members of the genus Enterovirus, family Picornaviridae, and order Picornavirales. E20_2010 library preparation was done using the Nextera XT DNA sample preparation kit, and paired-end sequencing (2 ϫ 301 bp) for 300 cycles was done using the MiSeq system (Illumina). Trimming and assembly were done using Geneious R10 software (7). E20_2014 library preparation was done using the Nextera DNA sample preparation kit.
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