Genome Sequences of Three Nocardia cyriacigeorgica Strains and One Nocardia asteroides Strain.

Florian Vautrin,Christophe Martin,Veronica Rodriguez-Nava,Thierry Winiarski,Benoit Cournoyer,Emmanuelle Bergeron,Danis Abrouk,Audrey Dubost,Petar Pujic,Vanessa Louzier

doi:10.1128/mra.00600-19

Abstract

We report four draft genome sequences of Nocardia spp. The strains are the Nocardia cyriacigeorgica DSM 44484 pathogenic type strain; two environmental isolates, Nocardia cyriacigeorgica EML446 and EML1456; and the Nocardia asteroides ATCC 19247 nonpathogenic type strain, with estimated genome sizes of 6.3 to 6.8 Mb. The study of these isolates will provide insight into physiology, evolution, and pathogenicity of Nocardia spp.

Highlights

Since Edmond Nocard first isolated Nocardia spp. in 1888 [1], 92 different species have been described [2]
Libraries with 2 ϫ 300-bp and 2 ϫ 125-bp reads were constructed for Biofidal and GATC, respectively
The reads were processed using Unicycler v0.4.3 [13], quality controls were assessed with FastQC and Trimmomatic, and contigs shorter than 200 bp were removed, resulting in genomes of 6.6 Mb for N. asteroides ATCC 19247T, 6.3 Mb for N. cyriacigeorgica DSM 44484T, 6.5 Mb for N. cyriacigeorgica EML446, and 6.8 Mb for N. cyriacigeorgica EML1456

Summary

Introduction

Since Edmond Nocard first isolated Nocardia spp. in 1888 [1], 92 different species have been described [2]. Size content No of No of BioProject no. N50 value Coverage No of % coding No of No of (Mbp) level (؋) CDSa proteins tRNAs rRNAs N. asteroides ATCC 19247T PRJNA542835 N. cyriacigeorgica EML1456 PRJNA542859 N. cyriacigeorgica EML446 PRJNA542857 N. cyriacigeorgica DSM 44484T PRJNA542831

Results

Conclusion