Abstract
We report here the sequences of two reference strains of parvovirus B19 (B19V) used for quantitation of B19V DNA. One reference strain has been established by the World Health Organization (WHO) and the other by the European Pharmacopeia (Ph. Eur.) and belong to B19V genotype 1a1 and 1a2, respectively.
Highlights
We report here the sequences of two reference strains of parvovirus B19 (B19V) used for quantitation of B19V DNA
The World Health Organization (WHO) has established an international standard (IS) and an international reference panel (IRP) comprising three different genotypes of B19V which have been used in the standardization of nucleic acid testing (NAT) assays for both blood/plasma safety as well as for clinical diagnostic purposes [2]
In the case of the WHO strain, 9 nucleotides upstream of the nonstructural protein (NS1) start codon, a polymorphism was identified with a T present in 87% of the reads and a C in the rest
Summary
We report here the sequences of two reference strains of parvovirus B19 (B19V) used for quantitation of B19V DNA. The World Health Organization (WHO) has established an international standard (IS) and an international reference panel (IRP) comprising three different genotypes of B19V which have been used in the standardization of NAT assays for both blood/plasma safety as well as for clinical diagnostic purposes [2]. The 1st and 2nd ISs correspond to the genotype 1 strain included in the 1st WHO IRP, and this represents the most predominant strain circulating worldwide.
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