Abstract
The genomes of two newly emerged Newcastle disease virus strains, chicken/Indonesia/Mega/001WJ/2013 and chicken/Indonesia/Cimanglid/002WJ/2015, from disease outbreaks in chickens in Indonesia are reported. Phylogenetic analysis of different genotypes of Newcastle disease virus using the F gene coding sequences suggests that these two strains belong to genotype VII.2, in class II of avian paramyxoviruses.
Highlights
Newcastle disease (ND) is one of the most severe infectious diseases of chickens
Sequence analysis revealed that the two strains differed in the amino acid sequence at the C terminus of the F protein cleavage site, which is a key determinant of ND virus (NDV)
The amino acid identities of NP, P, M, F, HN, and L proteins between the current virus strains and the LaSota vaccine strain that is most commonly used in Indonesia are 92%, 81%, 88%, 89%, 85%, and 94%, respectively
Summary
Newcastle disease (ND) is one of the most severe infectious diseases of chickens. The causative agent, ND virus (NDV), is a member of the avian genus Orthoavulavirus (subfamily Avulavirinae, family Paramyxoviridae) [1]. A gap in the sequence of Mega/001WJ was closed using reverse transcriptase PCR (Qiagen) and Sanger sequencing [13], and BioEdit v7.2 was used to merge sequences [14].
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