Genome Sequences of Listeria monocytogenes Strains with Resistance to Arsenic.

Vikrant Dutta,Eric Altermann,Dereje D Jima,Todd J Ward,Cameron Parsons,Sangmi Lee,Sophia Kathariou,Nathane Orwig

doi:10.1128/genomea.00327-17

Vikrant Dutta, Eric Altermann + Show 6 more

Open Access

https://doi.org/10.1128/genomea.00327-17

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Abstract

ABSTRACTListeria monocytogenes frequently exhibits resistance to arsenic. We report here the draft genome sequences of eight genetically diverse arsenic-resistant L. monocytogenes strains from human listeriosis and food-associated environments. The availability of these genomes will help elucidate the role of heavy-metal resistance in the ecology of L. monocytogenes.

Highlights

Listeria monocytogenes frequently exhibits resistance to arsenic
The genomic DNA extracted with the DNeasy blood and tissue kit (Qiagen, Valencia, CA) was used to prepare the sequencing libraries on a Zephyr next-generation sequencing (NGS) workstation (PerkinElmer, Waltham, MA) using the NEBNext Fast DNA library prep set (New England BioLabs, Ipswich, MA)
The Ion Torrent Personal Genome Machine was used for sequencing with an Ion 318 Chip version 2 and the Ion PGM 400 sequencing kit (Life Technologies, Inc., Grand Island, NY)

Summary

Introduction

Listeria monocytogenes frequently exhibits resistance to arsenic. We report here the draft genome sequences of eight genetically diverse arsenic-resistant L. monocytogenes strains from human listeriosis and food-associated environments. The genomic DNA extracted with the DNeasy blood and tissue kit (Qiagen, Valencia, CA) was used to prepare the sequencing libraries on a Zephyr next-generation sequencing (NGS) workstation (PerkinElmer, Waltham, MA) using the NEBNext Fast DNA library prep set (New England BioLabs, Ipswich, MA). The Ion Torrent Personal Genome Machine was used for sequencing with an Ion 318 Chip version 2 and the Ion PGM 400 sequencing kit (Life Technologies, Inc., Grand Island, NY).

Results

Conclusion