Year-end Sale % OFF 
Abstract
Frankiaceae are bacterial endosymbionts that are also found free-living in soil. Here, we present the genome sequences of two novel bacterial members of the order Frankiales, class Actinobacteria, isolated from temperate terrestrial forest soils. The genomes for MT45 and GAS493 indicate a genetic capacity for carbohydrate degradation but not nitrogen fixation.
Highlights
Frankiaceae are best known as spore-forming, nitrogen-fixing actinobacteria (1) capable of associating with actinorhizal plants (2), these plants are not required for their growth (3)
SMRTbell libraries were constructed and sequenced on the PacBio RS platform at the U.S Department of Energy (DOE) Joint Genome Institute (JGI) (6), generating 383,007 reads filtered to 122,552 subreads totaling 611.7 Mbp for MT45 and 506,493 reads filtered to 259,823 subreads totaling 914.4 Mbp for GAS493
Genes were identified using Prodigal (9), manually curated using GenePRIMP (10), and translated to search the NCBI nonredundant database and the UniProt, TIGRFam, Pfam, KEGG, Clusters of Orthologous Groups (COG), and InterPro databases. rRNA genes were found by searches against models of the rRNA genes built from SILVA (11)
Summary
Frankiaceae are best known as spore-forming, nitrogen-fixing actinobacteria (1) capable of associating with actinorhizal plants (2), these plants are not required for their growth (3). SMRTbell libraries were constructed and sequenced on the PacBio RS platform at the U.S Department of Energy (DOE) Joint Genome Institute (JGI) (6), generating 383,007 reads filtered to 122,552 subreads totaling 611.7 Mbp for MT45 and 506,493 reads filtered to 259,823 subreads totaling 914.4 Mbp for GAS493. The libraries had an N50 value of 4.229 Mb for MT45 and 4.88 Mb for GAS493. The final assembly was one 4.229-Mbp scaffold with 70.0ϫ input read coverage for MT45 and one 4.880-Mbp scaffold with 156.0ϫ coverage for GAS493.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
