Abstract
Presented are five genomes from the polyextremophilic (optimal temperature of >65°C and optimal pH of <3.5) archaeal family Sulfolobaceae, greatly expanding order-wide genomic diversity. Included are the only obligate anaerobic species, several facultative sulfur utilizers, two metal mobilizers, one facultative chemolithoautotroph with robust metabolic versatility, and some of the most thermophilic thermoacidophiles reported to date.
Highlights
Presented are five genomes from the polyextremophilic (optimal temperature of Ͼ65°C and optimal pH of Ͻ3.5) archaeal family Sulfolobaceae, greatly expanding order-wide genomic diversity
Presented are five genomes from the polyextremophilic archaeal family Sulfolobaceae, greatly expanding order-wide genomic diversity
Single-molecule real-time (SMRT) sequencing was performed with either a SMRT cell with the RS II system or multiplexing (Barcoded Adapter kit 8A) in a SMRT cell with the Sequel system (Pacific Biosciences, Menlo Park, CA, USA). Both library configurations were size selected with BluePippin (15 or 3 kb) and prepared with a SMRTbell template preparation kit 1.0-SPv3
Summary
Presented are five genomes from the polyextremophilic (optimal temperature of Ͼ65°C and optimal pH of Ͻ3.5) archaeal family Sulfolobaceae, greatly expanding order-wide genomic diversity. Single-molecule real-time (SMRT) sequencing was performed with either a SMRT cell with the RS II system or multiplexing (Barcoded Adapter kit 8A) in a SMRT cell with the Sequel system (Pacific Biosciences, Menlo Park, CA, USA). Both library configurations were size selected with BluePippin (15 or 3 kb) and prepared with a SMRTbell template preparation kit 1.0-SPv3.
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