Abstract
Five equine herpesvirus 1 (EHV-1) genome sequences with links to an EHV-1 outbreak with neurological disorders after a horse gathering in Valencia, Spain, in February 2021, were determined. All strains showed the closest relationships to strains from Belgium and the United Kingdom, indicating a common source of infection.
Highlights
Five equine herpesvirus 1 (EHV-1) genome sequences with links to an EHV-1 outbreak with neurological disorders after a horse gathering in Valencia, Spain, in February 2021, were determined
Viral DNA was extracted from the cell culture supernatant using the QuickDNA/RNA viral kit (Zymo Research) at PathoSense BV
Native EHV-1 DNA was sequenced on a MinION R.9.4 flow cell (FLO-MIN106; Oxford Nanopore Technologies [ONT]) using the ONT ligation sequencing protocol (LSK-109; ONT)
Summary
Five equine herpesvirus 1 (EHV-1) genome sequences with links to an EHV-1 outbreak with neurological disorders after a horse gathering in Valencia, Spain, in February 2021, were determined. Viral DNA was extracted from the cell culture supernatant using the QuickDNA/RNA viral kit (Zymo Research) at PathoSense BV. Native EHV-1 DNA was sequenced on a MinION R.9.4 flow cell (FLO-MIN106; Oxford Nanopore Technologies [ONT]) using the ONT ligation sequencing protocol (LSK-109; ONT). Raw data were processed using an in-house pipeline.
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