Genome Sequences of Chikungunya Virus Strains from Bangladesh and Thailand.

Alyssa T Pyke,Peter Burtonclay,Amanda De Jong,Jamie Mcmahon,Neelima Nair,Simon Roux

doi:10.1128/mra.01452-19

Abstract

We sequenced the genomes of two chikungunya virus isolates obtained from viremic patients who had traveled to Australia. The first patient acquired the infection in Bangladesh in 2017, and the second was infected in Thailand in 2019. Phylogenetic sequence analysis demonstrated that both isolates belonged to the East/Central/South African genotype.

Highlights

Within the last 20 years, the reemergence and global borne chikungunya virus (CHIKV) have resulted in explosive epidemics throughout the Pacific, Asia, and the Americas, affecting millions of people [1]
In July 2017, a febrile male patient traveled to Brisbane, Australia, from Bangladesh
RNA was converted to cDNA using the ProtoScript II first-strand cDNA kit (New England Biolabs), and second-strand cDNA synthesis was performed using an enzyme cocktail of Escherichia coli DNA ligase, DNA polymerase I, and RNase H

Summary

Introduction

Within the last 20 years, the reemergence and global borne chikungunya virus (CHIKV) (genus Alphavirus, spread family of the arthropodTogaviridae) have resulted in explosive epidemics throughout the Pacific, Asia, and the Americas, affecting millions of people [1]. In July 2017, a febrile male patient traveled to Brisbane, Australia, from Bangladesh. In September 2019, a female patient with fever and joint pains arrived in Brisbane after recent travel in Thailand.

Results

Conclusion