Genome Sequences of Both Organelles of the Grapevine Rootstock Cultivar 'Börner'.

Bianca Frommer,Prisca Viehöver,Ludger Hausmann,Bernd Weisshaar,Bruno Huettel,Daniela Holtgräwe,Reinhard Töpfer

doi:10.1128/mra.01471-19

Bianca Frommer, Prisca Viehöver + Show 5 more

Open Access

https://doi.org/10.1128/mra.01471-19

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Abstract

Genomic long reads of the interspecific grapevine rootstock cultivar 'Börner' (Vitis riparia GM183 × Vitis cinerea Arnold) were used to assemble its chloroplast and mitochondrion genome sequences. We annotated 133 chloroplast and 172 mitochondrial genes, including the RNA editing sites. The organelle genomes in 'Börner' were maternally inherited from Vitis riparia.

Highlights

Genomic long reads of the interspecific grapevine rootstock cultivar ‘Börner’ (Vitis riparia GM183 ϫ Vitis cinerea Arnold) were used to assemble its chloroplast and mitochondrion genome sequences
Indexed Illumina sequencing libraries were prepared from 1,000 ng total RNA according to the TruSeq RNA Sample Preparation v2 Guide
The resulting transcriptome sequencing (RNA-Seq) libraries were pooled in equimolar amounts and sequenced in a 2 ϫ 100-nt paired-end format on a HiSeq 1500 instrument

Summary

Introduction

Genomic long reads of the interspecific grapevine rootstock cultivar ‘Börner’ (Vitis riparia GM183 ϫ Vitis cinerea Arnold) were used to assemble its chloroplast and mitochondrion genome sequences. RNA was extracted from ‘Börner’ tissues using the peqGOLD plant RNA kit (Peqlab) according to the manufacturer’s instructions. Indexed Illumina sequencing libraries were prepared from 1,000 ng total RNA according to the TruSeq RNA Sample Preparation v2 Guide.

Results

Conclusion