Abstract
Complete genome sequences of six Ambystoma tigrinum viruses (ATV) were determined directly from tail clips of western tiger salamanders (Ambystoma mavortium) from 2013 (high-mortality year) and 2014 (low-mortality year) in Alberta, Canada. The genome lengths ranged from 106,258 to 106,915 bp and contained 108 open reading frames encoding predicted proteins larger than 50 amino acids.
Highlights
Ambystoma tigrinum virus (ATV), a virus with a double-stranded DNA genome of ϳ106 kb (1), and other emerging viruses of the genus Ranavirus and family Iridoviridae affect a wide range of amphibians, reptiles, and fish (2, 3)
The complete genomes were searched for open reading frames (ORFs), using the Geneious ORFfinder, with a minimum length of 150 nucleotides
Coding sequences (CDS) from 15 complete ATV genomes imported from NCBI (23 June 2017) were transferred to the six samples using the Geneious Live Annotate and Predict tool with a 65% nucleotide identity threshold
Summary
Ambystoma tigrinum virus (ATV), a virus with a double-stranded DNA (dsDNA) genome of ϳ106 kb (1), and other emerging viruses of the genus Ranavirus and family Iridoviridae affect a wide range of amphibians, reptiles, and fish (2, 3). Raw sequencing reads from each sample were mapped back to the assembled genomes in Geneious (v.3.11.4) (9) and manually corrected for errors in the assemblies. All samples had a GC content of 53.8% and had 108 ORFs encoding predicted proteins larger than 50 amino acids.
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