Abstract
Genome sequences for three strains of denitrifying bacteria (Alphaproteobacteria—Afipia sp. strain 1NLS2 and Hyphomicrobium denitrificans strain 1NES1; Firmicutes—Bacillus sp. strain 1NLA3E) isolated from the nitrate- and uranium-contaminated subsurface of the Oak Ridge Integrated Field Research Challenge (ORIFRC) site, Oak Ridge Reservation, TN, are reported.
Highlights
Genome sequences for three strains of denitrifying bacteria (Alphaproteobacteria—Afipia sp. strain 1NLS2 and Hyphomicrobium denitrificans strain 1NES1; Firmicutes—Bacillus sp. strain 1NLA3E) isolated from the nitrate- and uranium-contaminated subsurface of the Oak Ridge Integrated Field Research Challenge (ORIFRC) site, Oak Ridge Reservation, TN, are reported
We present the complete or nearly complete genomes of three additional denitrifying bacteria isolated from the ORIFRC site (2)
Nitrate and nitrite reductase genes were similar to those in strain ATCC 53690, but no nitric or nitrous oxide reductase genes were identified in ATCC 53690
Summary
Genome sequences for three strains of denitrifying bacteria (Alphaproteobacteria—Afipia sp. strain 1NLS2 and Hyphomicrobium denitrificans strain 1NES1; Firmicutes—Bacillus sp. strain 1NLA3E) isolated from the nitrate- and uranium-contaminated subsurface of the Oak Ridge Integrated Field Research Challenge (ORIFRC) site, Oak Ridge Reservation, TN, are reported. To better understand the denitrification capacity of the bacterial community, we isolated bacteria from the site under denitrifying conditions (2). Bacteria from five genera were isolated (Afipia, Hyphomicrobium, Rhodanobacter, Intrasporangium, and Bacillus). Genome sequences from Rhodanobacter isolates have been described previously (3).
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