Genome Sequence Resource of Bacillus mojavensis KRS009, a Rhizospheric Microorganism with Biocontrol Potential

Abstract

Bacillus mojavensis KRS009 was identified as antagonistic strain with strong inhibitory effect to various phytopathogenic fungi. To provide a further insight into its biocontrol mechanisms and ability to improve plant salt tolerance, the high-quality complete genome of KRS009 was sequenced and assembled using PacBio-HiFiReads. KRS009 genome consists of one circular chromosome as 4,089,687 bp with 43.5% GC content. 4,062 open reading frames, of which 3,920 protein-coding genes, 86 transfer RNA, 10 ribosomal RNA, and 26 small RNA were identified in this genome. Among them, genes related to compatible solutes, including treC, galU, proX, and proW, are involved in the synthesis of trehalose, proline, and betaine metabolism, play important role in relieving osmotic stress. In addition, the KRS009 genome contains genes associated with high salinity tolerance, including those responsible for Na+/H+ antiporters, K+ transporters TrkH thiamin phosphate synthase, K+-sensing histidine kinase, aryl-phospho-beta-D-glucosidase, flavoprotein CzcO associated with the cation diffusion facilitator CzcD and chaperonin GroEL. Together, the high-quality genome resource of strain KRS009 would provide molecular basis for further research on its biocontrol and plant salt tolerance mechanisms.