Abstract
Vibrio cholerae is a human bacterial pathogen and an inhabitant of aquatic environments. It is endemic to many regions of the world but is typically found in warm climates in saltwater. Here, we present the sequence of a V. cholerae strain isolated from a freshwater river in Ohio.
Highlights
Vibrio cholerae is a human bacterial pathogen and an inhabitant of aquatic environments
Genome sequencing of the strain was performed by SNPsaurus (Eugene, OR) using PacBio technology (Menlo Park, CA)
Sequences were assembled into three contigs using Canu v.1.7 (7), for a total of 4,139,713 bp
Summary
Vibrio cholerae is a human bacterial pathogen and an inhabitant of aquatic environments. To prepare for sequencing, a single colony of V. cholerae isolate D1 was grown overnight at 37°C on LB agar, and genomic DNA was isolated using the Qiagen QIAamp DNA minikit (Valencia, CA). Genome sequencing of the strain was performed by SNPsaurus (Eugene, OR) using PacBio technology (Menlo Park, CA).
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