Abstract
Mesorhizobium loti strain R88B was isolated in 1993 in the Rocklands range in Otago, New Zealand from a Lotus corniculatus root nodule. R88B is an aerobic, Gram-negative, non-spore-forming rod. This report reveals the genome of M. loti strain R88B contains a single scaffold of size 7,195,110 bp which encodes 6,950 protein-coding genes and 66 RNA-only encoding genes. This genome does not harbor any plasmids but contains the integrative and conjugative element ICEMlSymR7A, also known as the R7A symbiosis island, acquired by horizontal gene transfer in the field environment from M. loti strain R7A. It also contains a mobilizable genetic element ICEMladhR88B, that encodes a likely adhesin gene which has integrated downstream of ICEMlSymR7A, and three acquired loci that together allow the utilization of the siderophore ferrichrome. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
Highlights
Mesorhizobium loti strain R88B was first described in studies that culminated in the discovery of the M. loti strain R7A symbiosis island [1,2]
(See figure on previous page.) Figure 2 Phylogenetic tree showing the relationships of Mesorhizobium loti R88B with other root nodule bacteria based on aligned sequences of the 16S rRNA gene (1,290 bp internal region)
The M. loti strain R88B genome consists of a single chromosome of 7.2 Mb predicted to encode 7,016 genes
Summary
Mesorhizobium loti strain R88B was first described in studies that culminated in the discovery of the M. loti strain R7A symbiosis island [1,2]. The genes fhuBD that encode two of the three subunits of the ferrichrome ABC transporter were located downstream of ICEMladhR88B and were absent from the previously sequenced genome of M. loti strain MAFF303099. We present a summary classification and a set of general features for M. loti strain R88B together with the description of the complete genome sequence and annotation.
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