Abstract
Leucobacter salsicius M1-8T is a member of the Microbacteriaceae family within the class Actinomycetales. This strain is a Gram-positive, rod-shaped bacterium and was previously isolated from a Korean fermented food. Most members of the genus Leucobacter are chromate-resistant and this feature could be exploited in biotechnological applications. However, the genus Leucobacter is poorly characterized at the genome level, despite its potential importance. Thus, the present study determined the features of Leucobacter salsicius M1-8T, as well as its genome sequence and annotation. The genome comprised 3,185,418 bp with a G+C content of 64.5%, which included 2,865 protein-coding genes and 68 RNA genes. This strain possessed two predicted genes associated with chromate resistance, which might facilitate its growth in heavy metal-rich environments.
Highlights
The strain M1-8T (= KACC 21127T = JCM 16362T) is the type strain of the species Leucobacter salsicius [1], which was isolated from a Korean salt-fermented seafood known as “jeotgal” in Korean
The genomic analysis of L. salsicius M1-8T should help us to understand the molecular basis of adaptation to a chromium-contaminated environment
16S rRNA analysis A representative genomic 16S rRNA gene of strain M1-8T was compared with those obtained using NCBI BLAST [17] with the default settings
Summary
Several Leucobacter strains have been reported to possess chromate resistance [1,4,11]. There are 18 validly named Leucobacter species, but the only sequenced genomes in this genus were Leucobacter sp. The highest resistance to chromate (up to 300 mM K2CrO4) was observed in L. chromiiresistens, in vivo [13]. No information has been generated on genes related to the mechanism of chromate resistance. L. salsicius strain M1-8T has lower chromate resistance than L. chromiiresistens but it still exhibits moderate resistance (up to 10.0 mM Cr(VI)). The genomic analysis of L. salsicius M1-8T should help us to understand the molecular basis of adaptation to a chromium-contaminated environment. The present study determined the classification and features of Leucobacter salsicius strain M1-8T, as well as its genome sequence and gene annotations
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