Genome sequence of strain HIMB624, a cultured representative from the OM43 clade of marine Betaproteobacteria.

Megan J Huggett,Darin H Hayakawa,Michael S Rappé

doi:10.4056/sigs.2305090

Abstract

Strain HIMB624 is a planktonic marine bacterium within the family Methylophilaceae of the class Betaproteobacteria isolated from coastal seawater of Oahu, Hawaii. This strain is of interest because it is one of few known isolates from an abundant clade of Betaproteobacteria found in cultivation-independent studies of coastal seawater and freshwater environments around the globe, known as OM43. Here we describe some preliminary features of the organism, draft genome sequence and annotation, and comparative genomic analysis with one other sequenced member of this clade (strain HTCC2181). The 1,333,209 bp genome of strain HIMB624 is arranged in a single scaffold containing four contigs, and contains 1,381 protein encoding genes and 39 RNA genes.

Highlights

Strain HIMB624 was isolated from surface seawater of Kaneohe Bay, a subtropical bay on the northeastern shore of Oahu, Hawaii, via dilution to extinction culturing methods [1,2]
This strain is of interest because it belongs to a globally ubiquitous clade of aquatic bacterioplankton known as OM43, within the obligately methylotrophic family Methylophilaceae of the class Betaproteobacteria
The OM43 lineage was first described in 1997 from a 16S rRNA gene survey of coastal bacterioplankton from the Atlantic coast of the United States [3], and the first published report describing the isolation of OM43 strains via modified extinction to dilution culturing methods was reported in 2002 [1]

Summary

Introduction

Strain HIMB624 was isolated from surface seawater of Kaneohe Bay, a subtropical bay on the northeastern shore of Oahu, Hawaii, via dilution to extinction culturing methods [1,2]. The genome sequence of a member of the OM43 lineage was reported for a strain isolated from the Pacific coast of the United States (HTCC2181) [4].

Results

Conclusion