Genome Sequence of Prosthecochloris sp. Strain HL-130-GSB from the Phylum Chlorobi.

Vera Thiel,Stephan C Schuster,Rikky W Purbojati,Stephen Lindemann,Daniela I Drautz-Moses,Donald A Bryant

doi:10.1128/genomea.00538-17

Abstract

ABSTRACTThe genome of the green sulfur bacterium Prosthecochloris sp. strain HL-130-GSB, isolated from a cyanobacterial mat obtained from Hot Lake, a saline meromictic lake in Washington, USA, comprises 2,437,774 bp in a single contig. The genome is predicted to encode 2,565 proteins and contain 47 tRNA genes and 2 rRNA operons.

Highlights

The genome of the green sulfur bacterium Prosthecochloris sp. strain HL-130-GSB, isolated from a cyanobacterial mat obtained from Hot Lake, a saline meromictic lake in Washington, USA, comprises 2,437,774 bp in a single contig
The assembly used for genome analysis contained a single contig with a length of 2,437,774 bp, with an average GϩC content of 52% and mean coverage of 260-fold
AmphoraNet [11] identified all 31 phylogenetic marker genes, which confirmed the phylogenetic assignment of this organism to the Chlorobiaceae

Summary

Introduction

The genome of the green sulfur bacterium Prosthecochloris sp. strain HL-130-GSB, isolated from a cyanobacterial mat obtained from Hot Lake, a saline meromictic lake in Washington, USA, comprises 2,437,774 bp in a single contig. Strain HL-130-GSB was sequenced using a PacBio RSII instrument in one single-molecule real-time (SMRT) cell that yielded 927,499,821 bp (91,952 subreads) and was assembled with the HGAP3 [8] workflow in the SMRT Analysis 2.3.0 package (https://github.com/PacificBiosciences/smrtmake). The assembly used for genome analysis contained a single contig with a length of 2,437,774 bp, with an average GϩC content of 52% and mean coverage of 260-fold.

Results

Conclusion