Genome Sequence of Penicillium solitum RS1, Which Causes Postharvest Apple Decay.

Jiujiang Yu,Joan W Bennett,Verneta L Gaskins,Yanbin Yin,Guohua Yin,Daniel R Shelton,Guangxi Wu,Wayne M Jurick

doi:10.1128/genomea.00363-16

Abstract

Penicillium species cause postharvest decay, commonly known as blue mold, in pome fruits, such as apples and pears. To devise novel strategies to prevent and reduce economic losses during storage, the genome sequence of Penicillium solitum RS1 is reported here for the first time.

Highlights

Penicillium species cause postharvest decay, commonly known as blue mold, in pome fruits, such as apples and pears
To deepen our understanding of the genetic differences likely contributing to their virulence, spore germination, and mycotoxin production, the genome of the wildtype strain of P. solitum (RS1) was sequenced, assembled, and annotated for comparative genomics study with the more virulent species P. expansum R19 strain (2)
Spores of P. solitum RS1 were inoculated in potato dextrose broth (PDB) medium at a final concentration of 106/ml and incubated at 25°C for 7 days under constant shaking at 150 rpm in a temperature-controlled incubator

Summary

Introduction

Penicillium species cause postharvest decay, commonly known as blue mold, in pome fruits, such as apples and pears. Penicillium is a fungal genus composed of saprophytes and human pathogens and includes several plant-pathogenic species that infect pome fruits (1). Penicillium solitum RS1, isolated from decayed apples during storage in Oregon, is a less virulent species than Penicillium expansum (2). To deepen our understanding of the genetic differences likely contributing to their virulence, spore germination, and mycotoxin production, the genome of the wildtype strain of P. solitum (RS1) was sequenced, assembled, and annotated for comparative genomics study with the more virulent species P. expansum R19 strain (2).

Results

Conclusion