Abstract
Colonization of the intestine with Oxalobacter formigenes reduces urinary oxalate excretion and lowers the risk of forming calcium oxalate kidney stones. Here, we report the genome sequence of Oxalobacter formigenes SSYG-15, a strain isolated from a stool sample from a healthy Chinese boy.
Highlights
Oxalobacter formigenes, an anaerobe that is extremely sensitive to oxygen with substrate specificity for oxalate, was first isolated in 1985 by Milton J
Library preparation was performed with the SMRTbell template prep kit 1.0 (Pacific Biosciences, USA), followed by single-molecule real-time (SMRT) sequencing on the Pacific Biosciences RS II platform (5)
Genome annotation was completed by submission to the NCBI Prokaryotic Genome Automatic Annotation Pipeline (PGAAP)
Summary
Oxalobacter formigenes, an anaerobe that is extremely sensitive to oxygen with substrate specificity for oxalate, was first isolated in 1985 by Milton J. For genomic DNA extraction, a single colony was inoculated in ATCC medium 1352 broth and cultured in an anaerobic environment at 37°C until saturation. Genomic DNA was isolated using the MagAttract high-molecular-weight (HMW) DNA kit (Qiagen) according to the manufacturer’s instructions (for Gram-negative bacteria).
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