Abstract
Here, we describe LilHazelnut, a novel mycobacteriophage that infects Mycobacterium smegmatis mc2155. LilHazelnut is a cluster Q phage that shares 99% nucleotide identity with phage Giles, is 53,746 bp in length, and has a G+C content of 67.5%. LilHazelnut is a temperate Siphoviridae virus, as is typical of cluster Q family members.
Highlights
We describe LilHazelnut, a novel mycobacteriophage that infects Mycobacterium smegmatis mc2155
The E value cutoff used for BLASTP and HHPred was 10 eϪ4
Automatic genome annotation followed by manual refinement revealed that LilHazelnut is part of the cluster Q phages and is predicted to have no tRNAs/transfer-messenger RNA genes but has 85 protein-coding genes and 35 genes with assigned functions
Summary
We describe LilHazelnut, a novel mycobacteriophage that infects Mycobacterium smegmatis mc2155. The LilHazelnut genome was annotated using Glimmer v3.02 (4), GeneMark v2.5p (5), BLASTP v2.7.1 (https://blast.ncbi.nlm.nih.gov/), HHPred v3.0beta (6), and Phamerator (7). The E value cutoff used for BLASTP and HHPred was 10 eϪ4. Automatic genome annotation followed by manual refinement revealed that LilHazelnut is part of the cluster Q phages and is predicted to have no tRNAs/transfer-messenger RNA (tmRNA) genes but has 85 protein-coding genes and 35 genes with assigned functions.
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