Genome Sequence of Mariprofundus sp. Strain EBB-1, a Novel Marine Autotroph Isolated from an Iron-Sulfur Mineral.

Areli Lopez,Sabrina Estrada,Roman Barco,Benjamin J Tully,Theadora Dingmon,David Emerson,Marwin Fernandez,Senay Beraki,Pratixaben Savalia,Sondra Broomell,Ricardo Canela,Jan P Amend,Kenneth Nealson,Daniel Albino,Frank J Stewart

doi:10.1128/mra.00995-19

Abstract

Mariprofundus sp. strain EBB-1 was isolated from a pyrrhotite biofilm incubated in seawater from East Boothbay (ME, USA). Strain EBB-1 is an autotrophic member of the class Zetaproteobacteria with the ability to form iron oxide biominerals. Here, we present the 2.88-Mb genome sequence of EBB-1, which contains 2,656 putative protein-coding sequences.

Highlights

Members of the class Zetaproteobacteria are known for their ability to obtain energy from the oxidation of ferrous [Fe(II)] to ferric [Fe(III)] iron in microaerobic environments at circumneutral pH [1]
Library construction and sequencing were performed at the Single Cell Genomics Center at the Bigelow Laboratory for Ocean Sciences following their published protocol [10]
Library construction was performed using the Nextera XT (Illumina) reagents, following the manufacturer’s suggestions, except that DNA was purified using column cleanup kits (Qiagen) and size selected to 500 Ϯ 50 bp using BluePippin (Sage Science, Beverly, MA, USA)

Summary

Introduction

Members of the class Zetaproteobacteria are known for their ability to obtain energy from the oxidation of ferrous [Fe(II)] to ferric [Fe(III)] iron in microaerobic environments at circumneutral pH [1]. Library construction and sequencing were performed at the Single Cell Genomics Center at the Bigelow Laboratory for Ocean Sciences following their published protocol [10]. Library construction was performed using the Nextera XT (Illumina) reagents, following the manufacturer’s suggestions, except that DNA was purified using column cleanup kits (Qiagen) and size selected to 500 Ϯ 50 bp using BluePippin (Sage Science, Beverly, MA, USA).

Results

Conclusion