Genome Sequence of Listeria monocytogenes Strain HPB5415, Collected during a 2008 Listeriosis Outbreak in Canada.

Arthur W Pightling,Franco Pagotto

doi:10.1128/genomea.00637-15

Abstract

Listeria monocytogenes strain HPB5415—isolated from deli meat—was found in 2008 to have the same pulsed-field gel electrophoresis patterns as a clinical strain (08-5923). However, whether nucleotide differences (single nucleotide polymorphisms [SNPs]) exist between their genomes was not determined. We sequenced the L. monocytogenes strain HPB5415 genome and identified 52 SNPs relative to strain 08-5923.

Highlights

Listeria monocytogenes strain HPB5415—isolated from deli meat—was found in 2008 to have the same pulsed-field gel electrophoresis patterns as a clinical strain (08-5923)
Consumption of foods contaminated with L. monocytogenes can cause a severe life-threatening illness called listeriosis [1, 2]
In 2008, a listeriosis outbreak caused by contaminated deli meats encompassed seven Canadian provinces (Alberta, British Columbia, Manitoba, New Brunswick, Ontario, Quebec, and Saskatchewan), resulting in 57 confirmed clinical cases and 22 deaths

Summary

Introduction

Listeria monocytogenes strain HPB5415—isolated from deli meat—was found in 2008 to have the same pulsed-field gel electrophoresis patterns as a clinical strain (08-5923). Listeria monocytogenes is a Gram-positive pathogenic bacterium that naturally inhabits plant, soil, and surface water environments [1] but may be present in ready-to-eat foods. Consumption of foods contaminated with L. monocytogenes can cause a severe life-threatening illness called listeriosis [1, 2]. In 2008, a listeriosis outbreak caused by contaminated deli meats encompassed seven Canadian provinces (Alberta, British Columbia, Manitoba, New Brunswick, Ontario, Quebec, and Saskatchewan), resulting in 57 confirmed clinical cases and 22 deaths (http://www.health.gov.on.ca/en/public/publications/disease/docs/listeriosis_outbreak_epi_sum.pdf).

Results

Conclusion