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Abstract
The vaginal microbiota, in particular Lactobacillus species, play an important role in female health through modulation of immunity, countering pathogens and maintaining a pH below 4.7. We report the isolation and genome sequence of Lactobacillus pentosus strain KCA1 (formally known as L. plantarum) from the vagina of a healthy Nigerian woman. The genome was sequenced using Illumina GA II technology. The resulting 16,920,226 paired-end reads were assembled with the Velvet tool. Contigs were annotated using the RAST server, and manually curated. A comparative analysis with the available genomes of L. pentosus IG1 and L. plantarum WCFS1 showed that over 15% of the predicted functional activities are found only in this strain. The strain has a chromosome sequence of 3,418,159 bp with a G+C content of 46.4%, and is devoid of plasmids. Novel gene clusters or variants of known genes relative to the reference genomes were found. In particular, the strain has loci encoding additional putative mannose phosphotransferase systems. Clusters of genes include those for utilization of hydantoin, isopropylmalate, malonate, rhamnosides, and genes for assimilation of polyglycans, suggesting the metabolic versatility of L. pentosus KCA1. Loci encoding putative phage defense systems were also found including clustered regularly interspaced short palindromic repeats (CRISPRs), abortive infection (Abi) systems and toxin-antitoxin systems (TA). A putative cluster of genes for biosynthesis of a cyclic bacteriocin precursor, here designated as pentocin KCA1 (penA) were identified. These findings add crucial information for understanding the genomic and geographic diversity of vaginal lactobacilli.
Highlights
Lactobacilli have long been known as an important constituent of a healthy vaginal ecology
Aberrations in the vaginal microbiota can result in bacterial vaginosis (BV), and higher rates of BV have been found in black women [4,5], likely due to social and hygiene practices [6,7,8]
General Genome Features The draft genome sequence of Lactobacillus pentosus KCA1 consists of 3,418,159 nucleotide base pairs in 83 contigs
Summary
Lactobacilli have long been known as an important constituent of a healthy vaginal ecology. It has been shown that L. iners is often dominant in Caucasian and black African women [3]. Aberrations in the vaginal microbiota can result in bacterial vaginosis (BV), and higher rates of BV have been found in black women [4,5], likely due to social and hygiene practices [6,7,8]. We isolated a strain of Lactobacillus pentosus and designated it KCA1. Like a number of other vaginal Lactobacillus strains developed as probiotics, KCA1 was shown to produce biosurfactants, hydrogen peroxide (H2O2), and inhibit the growth of intestinal and urogenital pathogens [9], as well as exhibit varying degrees of acid and bile tolerance [10]
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