Abstract
The draft genome sequence of the green filamentous anoxygenic phototrophic (FAP) bacterium "Candidatus Viridilinea halotolerans" strain Chok-6, isolated from a cold saline sulfide-rich spring near Lake Chokrak, is presented. The genome sequence is annotated for elucidation of the taxonomic position of Chok-6 and to extend the public genome database.
Highlights
The draft genome sequence of the green filamentous anoxygenic phototrophic (FAP) bacterium “Candidatus Viridilinea halotolerans” strain Chok-6, isolated from a cold saline sulfide-rich spring near Lake Chokrak, is presented
Raw reads were quality checked with FastQC v. 0.11.7, and low-quality reads were trimmed with Trimmomatic v. 0.36 [10]
The average nucleotide identity (ANI) [18] and digital DNA-DNA hybridization [19] values of 81.3% and 27.6%, respectively, to the genome of the closest strain, “Ca. Viridilinea mediisalina” Kir15-3F, were below the criteria for assignment to separate species [20], which indicates that the strain Chok-6 belongs to a new Viridilinea species
Summary
The draft genome sequence of the green filamentous anoxygenic phototrophic (FAP) bacterium “Candidatus Viridilinea halotolerans” strain Chok-6, isolated from a cold saline sulfide-rich spring near Lake Chokrak, is presented. Genomic DNA was extracted using a DNeasy PowerSoil kit (Qiagen) according to the manufacturer’s instructions. Libraries were constructed with the NEBNext DNA library prep reagent set for Illumina, per the kit’s protocol. Sequencing was undertaken with the Illumina HiSeq 1500 platform with pair-end 230-bp reads.
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