Genome Sequence of Brevundimonas sp., an Arsenic Resistant Soil Bacterium

Javiera Soto,Giovanni Larama,Trevor C Charles,Michael D J Lynch,Hector Herrera,César Arriagada

doi:10.3390/d13080344

Javiera Soto, Giovanni Larama + Show 4 more

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https://doi.org/10.3390/d13080344

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Journal: Diversity	Publication Date: Jul 27, 2021
Citations: 10	License type: CC BY 4.0

Affiliation: Universidad de La Frontera, University of Waterloo

Abstract

Brevundimonas sp. is a bacteria able to grow in metal(loid) contaminated soil from Puchuncaví Valley, central Chile. This study has isolated a bacterial strain capable of growth under high doses of arsenic (As) (6000 mg L−1), and a draft genome sequence was generated. Additionally, real-time PCR was performed to examine the effect of As on some genes related to As resistance. Results demonstrated a total of 3275 predicted annotated genes with several genes related to the ars operon, metal(loid) resistance-related genes, metal efflux pumps, and detoxifying enzymes. Real-time PCR showed that the arsB involved in the efflux of As was down-regulated, whereas arsR, arsH, and ACR3 did not show differences with the addition of As. Our study provides novel evidence of diverse As regulating systems in tolerant bacteria that will lead to a better understanding of how microorganisms overcome toxic elements and colonize As contaminated soils and to the possible use of their specific properties in bioremediation.

Highlights

Arsenic (As) is a metalloid ubiquitous in the environment with origins from natural sources and anthropogenic activities [1]
The selected bacteria showed 100% identity with Brevundimonas intermedia (Accession number KR811205.1), the genome evaluated in autoMLST showed that the closest genomes in the NCBI database measured as Average Nucleotide Index (ANI) are from Brevundimonas genus, having Brevundimonas
The selected bacteria showed 100% identity with Brevundimonas intermedia (Accession number KR811205.1), the genome evaluated in autoMLST showed that the closest genomes in the NCBI database measured as Average Nucleotide Index (ANI) are from Brevundimonas genus, having Brevundimonas sp. assembly code GCF_001422455 the highest ANI of 90.5%

Summary

Introduction

Arsenic (As) is a metalloid ubiquitous in the environment with origins from natural sources and anthropogenic activities [1]. These activities include the burning of fossil fuels and the use of agricultural pesticides and fertilizers, which contribute to the abundance of As in the environment [2]. Inactivate key enzymes through interaction with sulfhydryl groups (-SH) or by replacement of ions from their active sites [9]. It has deleterious effects on carbohydrate and lipid metabolism, among others [6]

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