Abstract
ABSTRACTWe report the discovery and genome sequence of phage Adumb2043, a siphovirus infecting Arthrobacter globiformis, B2979-SEA. Adumb2043 was isolated from soil collected in Colorado Springs, Colorado. The genome has a length of 43,100 bp and contains 68 predicted protein-coding genes and no tRNA genes. Adumb2043 is related to actinobacteriophages Elezi and London.
Highlights
We report the discovery and genome sequence of phage Adumb2043, a siphovirus infecting Arthrobacter globiformis, B2979-SEA
Adumb2043 was isolated and purified from a soil sample collected in a public park in Colorado Springs, Colorado (38.89966N, 104.69988W), using an enrichment method outlined in the SEA-PHAGES manual [8], with PYCa medium at 30°C and the host A. globiformis B2979-SEA
Transmission electron microscopy analyses reveal Adumb2043 to have an icosahedral head and noncontractile flexible tail (Fig. 1), which are characteristic of the siphoviruses [9]
Summary
We report the discovery and genome sequence of phage Adumb2043, a siphovirus infecting Arthrobacter globiformis, B2979-SEA. Adumb2043 was isolated and purified from a soil sample collected in a public park (under grass) in Colorado Springs, Colorado (38.89966N, 104.69988W), using an enrichment method outlined in the SEA-PHAGES manual [8], with PYCa medium at 30°C and the host A. globiformis B2979-SEA. Genomic DNA extraction was performed at Colorado State University-Pueblo using the Promega Wizard DNA extraction kit, following the manufacturer’s instructions and the SEA-PHAGES manual [8].
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