Genome Sequence of Bacillus glycinifermentans TH008, Isolated from Ohio Soil.

Daniel R Zeigler

doi:10.1128/genomea.01573-15

Abstract

The genome sequence of an Ohio soil isolate, TH008, was determined. The sequence reveals a close relationship between TH008 and domesticated Bacillus glycinifermentans strains found in a traditional Korean fermented soybean food.

Highlights

To determine the genome sequence for TH008, an Illumina paired-end library (2 ϫ 150 bp) was sequenced
4 million read pairs were selected and sequence adapter trimming was performed followed by digital normalization [4]
De novo assembly was performed on the resulting normalized paired-end reads with the Velvet assembler [5], using a minimum contig length cutoff of 1,000 bp and k-mer length 77

Summary

Introduction

To determine the genome sequence for TH008, an Illumina paired-end library (2 ϫ 150 bp) was sequenced. 4 million read pairs were selected and sequence adapter trimming was performed followed by digital normalization [4]. De novo assembly was performed on the resulting normalized paired-end reads with the Velvet assembler [5], using a minimum contig length cutoff of 1,000 bp and k-mer length 77.

Results

Conclusion