Abstract
Arthrobacter phage Scuttle was isolated by enrichment from a dry soil sample (collected in Upper Darby, Pennsylvania) on host Arthrobacter sp. ATCC 21022. The genome of this phage is 43,729 bp long, has a GC content of 61.1%, and has 61 annotated protein-coding genes.
Highlights
Bacteriophages are regarded as the “dark matter” of the biosphere, due to their abundance in our environment [1]
All software was used with default settings
No tRNA or transfer-messenger RNA genes were detected by ARAGORN v1.2.38 [19] or tRNAscan-SE v2.0 [20]
Summary
Bacteriophages are regarded as the “dark matter” of the biosphere, due to their abundance in our environment [1]. Sequencing, assembly, and finishing of the genome were performed according to Russell [10]. The phage sequencing library was prepared using the New England BioLabs (NEB) Ultra II kit v3 and was sequenced using the Illumina MiSeq platform, generating 150-bp unpaired reads. Raw reads were assembled using Newbler 2.9 [11] with default settings, generating a single contig with a coverage of approximately 5,080-fold. Phage ends were determined as previously described [10] using Consed v29 [12] to check for completeness and accuracy of termini.
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