Genome Sequence of "Anthococcus," a Novel Genus of the Family Streptococcaceae Isolated from Flowers.

Li-Oon Chuah,Rosma Ahmad,Kwai Lin Thong,Min Tze Liong,Gulam Rusul,Ahamed Kamal Shamila-Syuhada,Kien-Pong Yap

doi:10.1128/genomea.01410-16

Li-Oon Chuah, Rosma Ahmad + Show 5 more

Open Access

https://doi.org/10.1128/genomea.01410-16

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Abstract

Here, we report the draft whole-genome sequence of “Anthococcus,” a novel genus of the family Streptococcaceae isolated from fresh flowers of a durian (Durio zibethinus) tree. The draft genome of Anthococcus sp. strain DF1 contains 2,157,756 bp, with a G+C content of 33.0%.

Highlights

Several novel lactic acid bacterial (LAB) species from the genera Fructobacillus, Lactobacillus, and Leuconostoc have been successfully isolated from flowers cultivated in temperate regions [1,2,3,4,5]
DF1 was first grown in all-purpose Tween (APT) broth (BD, Franklin Lakes, NJ, USA) supplemented with 1% sodium pyruvate at 30°C for 24 to 48 h, and genomic DNA extraction of the overnight cultures was performed using the DNeasy blood and tissue kit (Qiagen, Germantown, MD, USA)
The assembled contigs were subsequently annotated with Rapid Annotations Using Subsystems Technology (RAST) [9] and curated as previously described [10, 11]

Summary

Introduction

Several novel lactic acid bacterial (LAB) species from the genera Fructobacillus, Lactobacillus, and Leuconostoc have been successfully isolated from flowers cultivated in temperate regions [1,2,3,4,5]. “Anthococcus” represents a novel genus of Streptococcaceae, a family to other well-known genera, such as Lactococcus and Streptococcus, and Lactovum [8]. Biochemical, phenotypical, and phylogenetic studies (our unpublished data), Anthococcus can be classified into a novel genus of Streptococcaceae.

Results

Conclusion