Genome Sequence of an Alphabaculovirus Isolated from the Oak Looper, Lambdina fiscellaria, Contains a Putative 2-Kilobase-Pair Transposable Element Encoding a Transposase and a FLYWCH Domain-Containing Protein.

George F Rohrmann,Martin A Erlandson,David A Theilmann

doi:10.1128/genomea.00186-15

George F Rohrmann, Martin A Erlandson + Show 1 more

Open Access

https://doi.org/10.1128/genomea.00186-15

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Abstract

The genome sequence of an alphabaculovirus isolated from Lambdina fiscellaria indicated that it is a novel member of a group II lineage. A putative transposable element was identified that contained two genes, including a transposase ortholog. These genes were most closely related to genes of the pea aphid, Acyrthosiphon pisum.

Highlights

The genome sequence of an alphabaculovirus isolated from Lambdina fiscellaria indicated that it is a novel member of a group II lineage
The relatedness of baculovirus genomes is normally determined by combining PIF2 and LEF8 amino acid sequences
The sequence of both these genes from LafiNPV showed the closest relatedness to sequences from the group II lineage that includes Orgyia leucostigma NPV, Euproctis pseudoconspersa NPV, and Buzura suppressaria NPV, with amino acid sequence identities of 74 to 76% (PIF2) and 64% (LEF8), suggesting that it represents a new alphabaculovirus species

Summary

Introduction

The genome sequence of an alphabaculovirus isolated from Lambdina fiscellaria indicated that it is a novel member of a group II lineage. A partial lef[8] gene sequence indicated it was a member of a group II lineage of alphabaculoviruses and was significantly different from other members of that lineage (1). In order to further characterize this virus, the genome was sequenced and determined to be 157,989 bp, with a GϩC content of about 44%, and it contains 135 predicted open reading frames of Ն25 amino acids.

Results

Conclusion