Abstract

Deep sequencing analysis of the transcriptome of aSolanum phureja cv. Criolla Colombia plant with symptoms typical of avirus disease revealed an infection with potato virus V(PVV). The PVV-phureja genome comprises 9904 nt, exhibits 83% nucleotide identity with currently fully sequenced PVV isolates and contains one large ORF that codes for apolyprotein of 3065 residues flanked by 5' and 3' UTR of 217 and 448 nt, respectively. Phylogenetic analysis of the PVV-phureja polyprotein indicates that it is divergent with respect to most PVV isolates. This is the first complete PVV genome of an isolate infecting ahost different to S. tuberosum and, to this date, the only one from the South American Andes.

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