Abstract
ABSTRACTAcinetobacter lactucae OTEC-02 was isolated from hydrocarbon-contaminated soils. Whole-genome sequence analysis was performed to learn more about the strain’s ability to degrade different types of recalcitrant toxic monoaromatic hydrocarbons. The genome of this bacterium revealed its genomic properties and versatile metabolic features, as well as a complete prophage.
Highlights
Acinetobacter lactucae OTEC-02 was isolated from hydrocarbon-contaminated soils
Clusters of orthologous groups (COGs) were allocated using BLASTx against the COG database, and the hits were accepted with an E value of 1e10 (3)
The comparison between the OTEC-02 and A. lactucae NRRLB B-41902 strains showed that both genomes are very similar
Summary
Acinetobacter lactucae OTEC-02 was isolated from hydrocarbon-contaminated soils. Whole-genome sequence analysis was performed to learn more about the strain’s ability to degrade different types of recalcitrant toxic monoaromatic hydrocarbons. The OTEC-02 genome was sequenced using the Pacific Biosciences (PacBio RSII) single-molecule real-time (SMRT) sequencing platform. Four SMRT cells of a 15-to-20-kb insert library were sequenced; 168,961 reads, with an average read length of 10,793 bp, were used for the de novo genome assembly, which was completed using the program RS_HGAP_Assembly.3 with SMRT Portal Analysis version 2.3.0 (2). One contig of 3,981,712 bp was assembled with a coverage of 392ϫ. Clusters of orthologous groups (COGs) were allocated using BLASTx against the COG database, and the hits were accepted with an E value of 1e10 (3).
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