Abstract
This study reports the genome sequence of an isolated African swine fever (ASF) virus (VNUA-ASFV-05L1/HaNam) obtained at the fourth passage on pulmonary alveolar macrophages. The virus was isolated during a typical acute ASF outbreak in pigs in a northern province of Vietnam in 2020.
Highlights
In this study, we isolated a virulent strain (VNUA-African swine fever (ASF) virus (ASFV)-05L1/HaNam) from the spleen of a fattening pig that had succumbed to an acute infection
The single contig (VNUA-ASFV-05L1/HaNam) with a BLASTN similarity to an ASFV was aligned with a number of reference genomes using MAFFT v7.450 [11]
The pairwise comparison of average nucleotide identity (ANI) between ASFV genomes was performed by ANI Calculator [12]
Summary
We isolated a virulent strain (VNUA-ASFV-05L1/HaNam) from the spleen of a fattening pig that had succumbed to an acute infection. The next-generation sequencing was conducted by Apical Scientific Sdn Bhd (Selangor, Malaysia). The library was constructed with the NEBNext Ultra DNA library preparation kit, and the sequencing platform was an Illumina NovaSeq 150PE system. Primer sequences were removed from raw Illumina reads using BBDuk of the BBTools package (https://jgi.doe .gov/data-and-tools/bbtools).
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