Abstract
We report here the genome sequence of a Staphylococcus xylosus clinical isolate, strain SMA0341-04 (UGA5), which contains one chromosome and at least one plasmid. Notably, strain SMA0341-04 (UGA5) contains the tetracycline efflux major facilitator superfamily (MFS) transporter (tetK) gene.
Highlights
Staphylococcus xylosus, a commensal Gram-positive bacterium humans and animals [1, 2], is a ubiquitous bacterium that is present on the skin of naturally present in a wide range of foodstuffs [3, 4]
Annotations were completed at the Los Alamos National Lab (LANL) using an automated system using the Ergatis workflow manager v.2.0 [19] and in-house scripts
Of all the predicted genes, 2,437 are common between SMA0341-04 (UGA5) and the S. xylosus strain HKUOPL8 genomes, with 278 genes being unique to SMA0341-04 (UGA5)
Summary
Staphylococcus xylosus, a commensal Gram-positive bacterium humans and animals [1, 2], is a ubiquitous bacterium that is present on the skin of naturally present in a wide range of foodstuffs [3, 4]. Bacterial DNA was extracted from a pure culture using the UltraClean microbial DNA isolation kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions with minimal modifications. The library was prepared from 100 ng of bacterial DNA using the NEBNext Ultra DNA library prep kit for Illumina (New England Biolabs, Ipswich, MA).
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