Abstract
The Gila monster (Heloderma suspectum) is native to the Sonoran Desert. Metagenomic analyses of a Gila monster fecal sample revealed the presence of a small, circular, single-stranded DNA virus that is most closely related to a gemykrogvirus (family Genomoviridae) genome from caribou feces sharing 88% genome-wide pairwise identity.
Highlights
Monster Despite (Heloderma suspectum) is a venomous lizard native to significant activity in recovering a broad diversity of the Sonoran viruses associated with various reptiles [1] in the last decade, only viruses from the Adenoviridae family [2, 3] have been detected in Gila monsters to date
A contig of ϳ2,000 nt was identified as having similarity to the replication-associated protein (Rep) encoded by viruses in the family
The Gila monster-associated virus 1 (GmaV1) genome shares 88%, 75%, and 66% genome pairwise identity, respectively, with a caribou feces-associated genomovirus sampled in Canada (GenBank accession number KJ938717) [9], a bovine blood-associated genomovirus from Germany (LK931484) [10], and a sewage-associated genomovirus from a sewage oxidation pond in New Zealand (KJ547634) [11]
Summary
Monster Despite (Heloderma suspectum) is a venomous lizard native to significant activity in recovering a broad diversity of the Sonoran viruses associated with various reptiles [1] in the last decade, only viruses from the Adenoviridae family [2, 3] have been detected in Gila monsters to date. Circular viral DNA was amplified using rolling circle amplification (RCA) with the Illustra The RCA products were used to prepare a 2 ϫ 100-bp paired-end library and sequenced on a HiSeq 4000 instrument.
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