Genome Sequence of a Novel, Newly Identified Isolate of Guinea Pig Cytomegalovirus, the CIDMTR Strain

Mark R Schleiss,Nelmary Hernandez-Alvarado,Thiruvarangan Ramaraj,John A Crow

doi:10.1128/genomea.01052-13

Mark R Schleiss, Nelmary Hernandez-Alvarado + Show 2 more

Open Access

https://doi.org/10.1128/genomea.01052-13

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Abstract

The sequence of a newly discovered isolate of guinea pig cytomegalovirus (GPCMV), the CIDMTR strain, was determined. The 232,778-nucleotide genome was generally well conserved with that of the 22122 reference strain, although some regions of substantial sequence divergence allowed annotation of strain-specific open reading frames encoding putative immune modulation gene products.

Highlights

The sequence of a newly discovered isolate of guinea pig cytomegalovirus (GPCMV), the CIDMTR strain, was determined
GPCMV strain 22122, isolated by Hartley in 1957 (ATCC VR-682), has to date been the only strain available [3]. This strain was sequenced using DNA purified both from tissue culture (TC)-passaged virus [4] and from guinea pig salivary gland (SG) homogenates following in vivo passage, with only minor differences noted [5]
SG homogenate was passaged in vivo in seronegative strain 2 guinea pigs

Summary

Introduction

The sequence of a newly discovered isolate of guinea pig cytomegalovirus (GPCMV), the CIDMTR strain, was determined. Guinea pig cytomegalovirus (GPCMV) provides a useful model for congenital human cytomegalovirus (HCMV) infection [1, 2]. This strain was sequenced using DNA purified both from tissue culture (TC)-passaged virus [4] and from guinea pig salivary gland (SG) homogenates following in vivo passage, with only minor differences noted [5]. Some genomic instability of strain 22122 has been noted [6, 7], and its isolation and passage history are incompletely defined.

Results

Conclusion