Abstract
We discovered a novel iflavirus from the transcriptome of the Bombyx mori pupa inoculated with the insect-pathogenic fungus Cordyceps militaris. The assembled iflavirus genome has 10,119 nucleotides, with a 3′-polyadenylated tail, and it encodes a polyprotein composed of 3,004 amino acids.
Highlights
The domestic silkworm Bombyx mori is well studied and has been of interest due to its excellent characteristic as a textile fiber; recently, it has been used as a host for the expression of eukaryotic proteins
The RNA sequencing (RNA-seq) analysis of B. mori inoculated with C. militaris detected an iflavirus genome sequence
We sequenced the mRNA of B. mori (Kinsyu-Showa strain) (Nichihara Research & Development Laboratories, Inc.) inoculated with C. militaris (NBRC 100741)
Summary
The domestic silkworm Bombyx mori is well studied and has been of interest due to its excellent characteristic as a textile fiber; recently, it has been used as a host for the expression of eukaryotic proteins. The pupa of B. mori was used as a host of the insect-pathogenic fungus Cordyceps militaris. The RNA sequencing (RNA-seq) analysis of B. mori inoculated with C. militaris detected an iflavirus genome sequence.
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