Abstract
ABSTRACTWe report here the complete genome sequence of a virus isolated from a diseased marbled eel (Anguilla marmorata) in Taiwan. The virus has been characterized as being related to Japanese eel endothelial cell-infecting virus (JEECV), with a large T-antigen-like protein. The sequence of the marbled eel virus displays low homology to the JEECV.
Highlights
We report here the complete genome sequence of a virus isolated from a diseased marbled eel (Anguilla marmorata) in Taiwan
The DNA was further extracted using a WelPrep DNA kit by Welgene and sonicated using a Misonix 3000 sonicator for sizes ranging from 400 to 500 bp, which were performed with a bioanalyzer DNA 1000 chip (Agilent Technologies)
Cleaned and filtered nuclear reads were assembled de novo using ABySS [9]
Summary
We report here the complete genome sequence of a virus isolated from a diseased marbled eel (Anguilla marmorata) in Taiwan. MEPyV (AMV-6) was proliferated in EK-1 cells, and the DNA was isolated and purified by a modification of the Hirt method [7] and delivered to Welgene Biotech (Taipei, Taiwan) for next-generation sequencing. The DNA was further extracted using a WelPrep DNA kit by Welgene and sonicated using a Misonix 3000 sonicator for sizes ranging from 400 to 500 bp, which were performed with a bioanalyzer DNA 1000 chip (Agilent Technologies).
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