Abstract

Verticillium nonalfalfae, a cosmopolitan soil-borne phytopathogen, causes vascular wilt in agricultural crops and perennial woody plants. Select strains of V. nonalfalfae can cause lethal disease in the invasive tree Ailanthus altissima and several have since been utilized as a biological control (biocontrol) against this widespread invader. Here, we report the genome sequence and annotation of V. nonalfalfae strain VnAa140/NRRL 66861.

Highlights

  • Verticillium nonalfalfae, a cosmopolitan soil-borne phytopathogen, causes vascular wilt in agricultural crops and perennial woody plants

  • Assembly (n ϭ 630; N50, 172 kb; max contig, 678 kb; GϩC content, 55%) from the Velvet contigs and 454 reads [16]. This assembly was cleaned of vector contamination and redundant contigs by Automatic Assembly for the Fungi (AAFTF; v0.2.1) [17] and was further corrected by 5 rounds of polishing with Pilon (v1.22) using the Illumina reads

  • Genome annotation was performed with funannotate (v1.5.0-760de7c) [18] utilizing available Verticillium dahliae and V. alfalfae transcripts and proteins as evidence [13, 19]

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Summary

Introduction

Verticillium nonalfalfae, a cosmopolitan soil-borne phytopathogen, causes vascular wilt in agricultural crops and perennial woody plants. Received 28 November 2018 Accepted 15 December 2018 Published 24 January 2019 mra.asm.org 1 Assembly (n ϭ 630; N50, 172 kb; max contig, 678 kb; GϩC content, 55%) from the Velvet contigs and 454 reads [16].

Results
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