Genome Sequence Databases: Genomic, Construction of Libraries

Abstract

A genomic library is a collection of overlapping segments of genomic DNA, cloned into a backbone vector, which statistically includes all regions of the genome of an organism. The resulting cloned DNA is then transformed into a suitable host cell line. Construction of a genomic library is an important initial step in many genetic studies or in the isolation and cloning of genes from an organism. Screening of genomic libraries has been useful in identifying genes of interest to the medical field and the biotechnology industry as well as in finding genes related to particular cellular functions. Additionally, creating a representative genomic library of an organism is a prerequisite for genomic mapping or complete genome sequencing. The success of a study involving genomic libraries is dependent upon the quality and features of the library. These features typically include the vector backbone used, the size of the genomic DNA insert, and the number of recombinant clones contained within the library.