Abstract
The genome of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was sequenced by 454 pyrosequencing technology. The size of the genome is 120,420 bp with 36.8% G+C content. It contains 127 hypothetical open reading frames (ORFs) covering 90.7% of the genome and includes the 37 conserved baculovirus core genes, 84 genes found in other baculoviruses, and 6 unique ORFs. No typical baculoviral homologous repeats (hrs) were present but the genome contained a region of repeated sequences. Gene Parity Plots revealed a 28.8 kb region conserved among the alpha- and beta-baculoviruses. Overall comparisons of BusuNPV to other baculoviruses point to a distinct species in group II Alphabaculovirus.
Highlights
The Baculovirdae is an insect-specific family of viruses with double stranded circular DNA genomes of 80 kb –180 kb
In this manuscript we report the complete genome of Buzura suppressaria nucleopolyhedrovirus (BusuNPV)
open reading frames (ORFs), 37 have been identified as core genes that exist in all sequenced baculoviruses and are essential for the viral life cycle
Summary
The Baculovirdae is an insect-specific family of viruses with double stranded circular DNA genomes of 80 kb –180 kb. A physical map of viral DNA was determined [12] and about 43.5 kb dispersed regions of the genome have been sequenced showing a distinct gene arrangement of BusuNPV Sequence analysis showed that BusuNPV is a group II Alphabaculovirus with a genome distinct from other so far sequenced baculoviruses.
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