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Abstract
With antimicrobial resistance on the rise, the discovery of new compounds with novel structural scaffolds exhibiting antimicrobial properties has become an important area of research. Such compounds can serve as starting points for the development of new antimicrobials. In this report, we present the draft genome sequence of the Zooshikella ganghwensis strain VG4, isolated from Red Sea sediments, that produces metabolites with antimicrobial properties. A genomic analysis reveals that it carries at least five gene clusters that have the potential to direct biosynthesis of bioactive secondary metabolites such as polyketides and nonribosomal peptides. By using in-silico approaches, we predict the structure of these metabolites.
Highlights
The emergence and spread of resistance against known antimicrobials has renewed interest in the discovery of microbial natural products with antimicrobial properties
The bacterial cultures were centrifuged to pellet the cells, and the remaining C6-Acyl Homoserine Lactone (AHL) in the culture supernatant were detected by adding it to the wells of the LB agar plate overlaid with C. violaceum
Different bioinformatic approaches have been developed for identifying such enzymes in the genomes, and for predicting the structures of polyketides (PK) and nonribosomal peptides (NRP) produced by these enzyme [23,24]
Summary
The emergence and spread of resistance against known antimicrobials has renewed interest in the discovery of microbial natural products with antimicrobial properties. Recent studies have revealed that microbes found in the Red Sea can produce a variety of antimicrobial compounds [1,2,3,4]. The sequencing of microbial genomes has revealed the immense genetic potential of microbes to synthesize bioactive secondary metabolites [5]; the vast majority of secondary metabolites has remained unidentified [6]. While doing the initial screening, we observed that the culture supernatant of one isolate could kill the biosensor strain Chromobacter violaceum CV026 used in the assay (Fig. 1). We hypothesized that this isolate produced secondary metabolites with antimicrobial properties. The red pigment was identified as Prodigiosin, which has shown anticancer and antimicrobial properties [9,10]
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