Abstract
Fragaria vesca is a species of diploid strawberry being developed as a model for the octoploid garden strawberry. This work sequenced and compared the genomes of three F. vesca accessions: ‘Hawaii 4′, ‘Rügen’, and ‘Yellow Wonder’. Genome-scale analyses of shared and distinct SNPs among these three accessions have revealed that ‘Rügen’ and ‘Yellow Wonder’ are more similar to each other than they are to ‘Hawaii 4’. Though all three accessions are inbred seven generations, each accession still possesses extensive heterozygosity, highlighting the inherent differences between individual plants even of the same accession. The identification of the impact of each SNP as well as the large number of Indel markers provides a foundation for locating candidate mutations underlying phenotypic variations among these F. vesca accessions and for mapping new mutations generated through forward genetics screens. Through systematic analysis of SNP variants affecting genes in anthocyanin biosynthesis and regulation, a candidate SNP in FveMYB10 was identified and then functionally confirmed to be responsible for the yellow color fruits made by many F. vesca accessions. As a whole, this study provides further resources for F. vesca and establishes a foundation for linking traits of economic importance to specific genes and variants.
Highlights
Fragaria vesca (F. vesca) is a diploid species of wild strawberry that has been cultivated in European gardens for centuries
FaMYB10 is expressed during the ripening stage of the receptacle fruit, and its expression was shown to be repressed by auxin and stimulated by ABA24
RNAi down-regulation of FaMYB10 resulted in significant reduction of anthocyanin in the receptacle fruit mediated by the reduced expression of biosynthesis genes at both early and late stages in the anthocyanin biosynthesis pathway[24]
Summary
Fragaria vesca (F. vesca) is a diploid species of wild strawberry that has been cultivated in European gardens for centuries. Transcriptome profiling in red Rü and yellow YW receptacle fruit showed that transcript levels of several anthocyanin biosynthesis enzyme genes (C4H, CHS, CHI, F3H, DFR, and ANS) and several MYB genes (MYB1, MYB86 and MYB39) were reduced in the YW fruit[9,27]. These differentially expressed genes may reflect downstream effects of the causal mutation, and none of the studies conducted functional tests of DNA variants that may exist between yellow and red accessions
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