Abstract
The genus Natronomonas contains two species, one haloalkaliphile (N. pharaonis) and one neutrophile (N. moolapensis). Here, we report the genome sequence of N. moolapensis strain 8.8.11. The overall genome properties are similar for the two species. Only the neutrophile contains bacteriorhodopsin and a membrane glycolipid.
Highlights
The two species currently assigned to the genus Natronomonas are N. pharaonis, a haloalkaliphile isolated from the Wadi Natrun in Egypt [1] [2], and N. moolapensis, a neutrophile that grows optimally at a pH of 7 to 7.5 and was recovered from a solar saltern in southeastern Australia [3]
The genome was annotated according to N. pharaonis and other haloarchaeal genomes maintained at a high annotation quality level within the HaloLex framework [6]
Several enzymes are found in N. moolapensis but not in N. pharaonis
Summary
The two species currently assigned to the genus Natronomonas are N. pharaonis, a haloalkaliphile isolated from the Wadi Natrun in Egypt [1] (which was previously sequenced) [2], and N. moolapensis, a neutrophile that grows optimally at a pH of 7 to 7.5 and was recovered from a solar saltern in southeastern Australia [3]. The genome of N. moolapensis strain 8.8.11 (JCM14361T ϭ DSM18674T) was sequenced by the same strategy as described recently [4]. Contigs from standard 454 whole-genome sequencing (171.4 Mb of raw data, assembled by Newbler into 118 contigs with 2.85 Mb and average coverage of 60.1-fold) were ordered by end-paired Sanger sequencing of cosmid clones (2,857 cosmid end sequences). There is a single chromosome of 2.9 Mb with a GϩC content of 64.5%, a single rRNA operon, 46 tRNA genes, and no plasmids.
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