Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074.

Eduardo Almeida,Stephen Jackson,Laurence Jennings,Alan Dobson,Andrés Felipe Carrillo Rincón,Navdeep Kaur,Olivier Thomas

doi:10.3390/microorganisms7100394

Eduardo Almeida, Stephen Jackson + Show 5 more

Open Access

https://doi.org/10.3390/microorganisms7100394

Copy DOI

Abstract

Much recent interest has arisen in investigating Streptomyces isolates derived from the marine environment in the search for new bioactive compounds, particularly those found in association with marine invertebrates, such as sponges. Among these new compounds recently identified from marine Streptomyces isolates are the octapeptidic surugamides, which have been shown to possess anticancer and antifungal activities. By employing genome mining followed by an one strain many compounds (OSMAC)-based approach, we have identified the previously unreported capability of a marine sponge-derived isolate, namely Streptomyces sp. SM17, to produce surugamide A. Phylogenomics analyses provided novel insights on the distribution and conservation of the surugamides biosynthetic gene cluster (sur BGC) and suggested a closer relatedness between marine-derived sur BGCs than their terrestrially derived counterparts. Subsequent analysis showed differential production of surugamide A when comparing the closely related marine and terrestrial isolates, namely Streptomyces sp. SM17 and Streptomyces albidoflavus J1074. SM17 produced higher levels of surugamide A than S. albidoflavus J1074 under all conditions tested, and in particular producing >13-fold higher levels when grown in YD and 3-fold higher levels in SYP-NaCl medium. In addition, surugamide A production was repressed in TSB and YD medium, suggesting that carbon catabolite repression (CCR) may influence the production of surugamides in these strains.

Highlights

Members of the Streptomyces genus are widely known to be prolific producers of natural products
In the SM17 culture in SYP-NaCl, surugamide A accounted for 10.60% (w/w) of the extract, compared to 2.44% and 1.13% from TSB and YD, respectively; while in J1074 it accounted for 3.55% (w/w) of the extract from the SYP-NaCl culture, compared to 0.27% and 0.09% from TSB and YD, respectively (Table 1). These results provide further insights into factors that are potentially involved in regulation the biosynthesis of surugamide A, in the albidoflavus phylogroup and in Streptomyces sp
This study identified the previously unreported capability of the marine sponge-derived isolate Streptomyces sp

Summary

Introduction

Members of the Streptomyces genus are widely known to be prolific producers of natural products Many of these compounds have found widespread use in the pharmaceutical industry as antibiotics, immunosuppressant, antifungal, anticancer, and anti-parasitic drugs [1]. We genetically characterised 13 Streptomyces spp. that were isolated from both shallow and deep-sea sponges, which displayed antimicrobial activities against a number of clinically relevant bacterial and yeast species [12,13]. SM17 demonstrated an ability to inhibit the growth of E. coli NCIMB 12210, methicillin-resistant S. aureus (MRSA), and Candida spp., when employing deferred antagonism assays [12,13]

Methods

Results

Conclusion