Genome mining and description of Streptomyces albidus sp. nov., an endophytic actinobacterium with antibacterial potential.

Abstract

An endophytic actinobacterium, strain CAP215T was isolated from the root sample of a native pine tree (Callitris preissii), Adelaide, South Australia. This strain was a Gram stain-positive, aerobic actinobacterium with well-developed substrate mycelia. It produced spiral chains ofspores. The closest phylogenetic members which shared the highest 16S rRNA gene sequence similarity were Streptomyces marinus DSM 41968T, Streptomyces haliclonae DSM 41970T and Streptomyces karpasiensis K413T at 98.2%, 98.0% and 97.9%, respectively. The major cellular fatty acid of this strain was anteiso-C15:0 and major menaquinone was MK-9(H4). Polar lipids of strain CAP215T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol dimannoside and two unknown glycolipids. Chemotaxonomic data confirmed strain CAP215T belonged to the genus Streptomyces. Genome of strain CAP215T was 6.65 Mb with 69.8% DNA G + C content and contains 5992 coding sequences (CDS). Biosynthesis Genes Clusters (BGCs) comprised post-translationally modified peptides (RiPPs) cluster, genes encoding enzyme relating to antibiotic production; actinorhodin, surfactin and tetracenomycin. Genome mining of this strain identified genes encoding proteins relating to plant growth promotion such as pyrophosphatase, ectoine synthase, superoxide dismutase and siderophore production; penibactin and desferrioxamine E. Genes encoding beneficial enzymes; amylase, β-xylosidase, chitinase, lipase and protease were detected. Thegenome of this strain contained genes encoding enzymes degrading xenobiotic compounds such as 2,4-dichlorophenol 6-monooxygenase, nitroreductase and epoxide hydrolase. Also, genes encoding squalene, hopene and betacarotenoid production were observed. Digital DNA-DNA (dDDH) hybridization, Average Nucleotide IdentityBLAST (ANIb), ANI-MUMmer (ANIm) between strain CAP215T and S. marinus DSM 41968T were 25.4 %, 82% and 86.4%, respectively. The data on the genotypic and phenotypic characteristics and genome analysis recognized the differentiation of strain CAP215T with the closest species with valid names. The name Streptomyces albidus sp. nov. was proposed forwhich the type strain is CAP215T (= DSM 42025T = NRRL B-24815T).